Peripherin Monoclonal antibody

Peripherin Monoclonal Antibody for WB, IHC, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig

Applications

WB, IHC, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

1H10B3

Cat no : 66317-1-Ig

Synonyms

PRPH, NEF4, 1H10B3



Tested Applications

Positive WB detected inrat brain tissue, human colon tissue, rat colon tissue
Positive IHC detected inhuman brain tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66317-1-Ig targets Peripherin in WB, IHC, ELISA, FC (Intra) applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peripherin fusion protein Ag11116
Full Name peripherin
Calculated Molecular Weight 470 aa, 54 kDa
Observed Molecular Weight 50-54 kDa
GenBank Accession NumberBC032703
Gene Symbol Peripherin
Gene ID (NCBI) 5630
RRIDAB_2881698
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Peripherin antibody 66317-1-IgDownload protocol
IHC protocol for Peripherin antibody 66317-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols