Peripherin Monoclonal antibody, PBS Only (Detector)

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  WB analysis using 66317-1-Ig (same clone as 66317-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66317-1-Ig (same clone as 66317-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

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  WB analysis of human colon using 66317-1-Ig (same clone as 66317-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat colon using 66317-1-Ig (same clone as 66317-1-PBS)

  rat colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66317-1-Ig (same clone as 66317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66317-1-Ig (same clone as 66317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66317-1-Ig (same clone as 66317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66317-1-Ig (same clone as 66317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

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  FC experiment of PC-12 using 66317-1-Ig (same clone as 66317-1-PBS)

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (66317-1-Ig, Clone:1H10B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51128-2

  Cytometric bead array standard curve of MP51128-2, Peripherin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66317-4-PBS. Detection antibody: 66317-1-PBS. Standard:Ag11116. Range: 0.781-100 ng/mL.

Peripherin Monoclonal Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 66317-1-PBS

Clone No.1H10B3

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

1H10B3, NEF4, PRPH

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Product Information

66317-1-PBS targets Peripherin as part of a matched antibody pair:

MP51128-2: 66317-4-PBS capture and 66317-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11116

  Product name: Recombinant human Peripherin protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 155-470 aa of BC032703

  Sequence: LELLGRERDRVQVERDGLAEDLAALKQRLEEETRKREDAEHNLVLFRKDVDDATLSRLELERKIESLMDEIEFLKKLHEEELRDLQVSVESQQVQQVEVEATVKPELTAALRDIRAQYESIAAKNLQEAEEWYKSKYADLSDAANRNHEALRQAKQEMNESRRQIQSLTCEVDGLRGTNEALLRQLRELEEQFALEAGGYQAGAARLEEELRQLKEEMARHLREYQELLNVKMALDIEIATYRKLLEGEESRISVPVHSFASLNIKTTVPEVEPPQDSHSRKTVLIKTIETRNGEVVTESQKEQRSELDKSSAHSY

• Full Name: peripherin
• Calculated Molecular Weight: 470 aa, 54 kDa
• Observed Molecular Weight: 50-54 kDa
• GenBank Accession Number: BC032703
• Gene Symbol: Peripherin
• Gene ID (NCBI): 5630
• RRID: AB_2881698
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P41219
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Peripherin is a neuron-specific intermediate filament protein that is particularly expressed in peripheral nervous system. It is used to study peripheral nerve development and regeneration, as it is highly expressed during elongation of axon. Peripherin is a routine marker for unmyelinated neurons in trigeminal ganglion (TG) and the dorsal root ganglion (DRG). Peripherin is also expressed in peri-insular areas of islets and it has been identified as a key autoantigen for type 1A diabetes.