CoraLite® Plus 647-conjugated Phospho-ATG4B (Ser383) Recombinant monoclonal antibody

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• CoraLite® Plus 647-conjugated Phospho-ATG4B (Ser383) Recombinant monoclonal antibody (CL647-85703)

Phospho-ATG4B (Ser383) Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL647-85703

Clone No.242927A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

ATG4B, APG4B, AUTL1, AUT-like 1 cysteine endopeptidase, Autophagin-1

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  FC experiment of K-562 using CL647-85703

  1X10^6 K-562 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-Atg4b (Ser383) Recombinant Antibody (CL647-85703, Clone:242927A2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-85703 targets Phospho-ATG4B (Ser383) in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: ATG4 autophagy related 4 homolog B (S. cerevisiae)
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44-48 kDa
• GenBank Accession Number: BC000719
• Gene Symbol: ATG4B
• Gene ID (NCBI): 23192
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y4P1
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATG4B stimulates autophagy by promoting autophagosome formation through reversible modification of ATG8. In humans, microtubule-associated protein 1 light chain, LC3B, is the best-characterized ATG8 isoform, and ATG4B, but not three other ATG4 isoforms, displays a highly selective preference toward LC3B. MST4 phosphorylates ATG4B at serine residue 383, which stimulates ATG4B activity and increases autophagic flux. (PMID: 29232556, PMID: 36056541)