Phospho-ATG4B (Ser383) Recombinant antibody

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  WB analysis using 85703-1-RR

  Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 85703-1-RR (Phospho-ATG4B (Ser383) antibody) blocked with BSA only, Phospho-ATG4B (Ser383) peptide or non-Phospho peptide at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-ATG4B (Ser383) Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85703-1-RR

Clone No.242927A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

ATG4B, APG4B, AUTL1, AUT-like 1 cysteine endopeptidase, Autophagin-1

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85703-1-RR targets Phospho-ATG4B (Ser383) in applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: ATG4 autophagy related 4 homolog B (S. cerevisiae)
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44-48 kDa
• GenBank Accession Number: BC000719
• Gene Symbol: ATG4B
• Gene ID (NCBI): 23192
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y4P1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG4B stimulates autophagy by promoting autophagosome formation through reversible modification of ATG8. In humans, microtubule-associated protein 1 light chain, LC3B, is the best-characterized ATG8 isoform, and ATG4B, but not three other ATG4 isoforms, displays a highly selective preference toward LC3B. MST4 phosphorylates ATG4B at serine residue 383, which stimulates ATG4B activity and increases autophagic flux. (PMID: 29232556, PMID: 36056541)