CoraLite® Plus 647-conjugated Phospho-EGFR (Tyr869) Recombinant monoclonal antibody

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• CoraLite® Plus 647-conjugated Phospho-EGFR (Tyr869) Recombinant monoclonal antibody (CL647-84839)

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  FC experiment of A431 using CL647-84839

  1X10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.13 ug Coralite® Plus 647 Phospho-EGFR (Tyr869) Recombinant Antibody (CL647-84839, Clone:241785G5)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-EGFR (Tyr869) Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL647-84839

Clone No.241785G5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

EGFR (Tyr845), EGFR Tyr845, p-EGFR, p-EGFR (Tyr845), p-EGFR Tyr845

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Tested Applications

• Positive FC (Intra) detected in: EGF 100ng/mL 30min A431 cells

This antibody, also known as EGFR Tyr845, is the site where the signal peptide is removed.

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-84839 targets Phospho-EGFR (Tyr869) in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Calculated Molecular Weight: 1210 aa, 134 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC094761
• Gene Symbol: EGFR
• Gene ID (NCBI): 1956
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00533
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Epidermal growth factor receptor (EGFR) is a member of the receptor tyrosine kinase (RTK) family and plays a critical role in a wide variety of cellular functions, including proliferation, differentiation and apoptosis. After EGFR homodimerization with extracellular ligands such as epidermal growth factor (EGF), the intracellular tyrosine kinase domain is activated and autophosphorylates multiple tyrosine residues in the C‐terminal tail domain. (PMID: 23822636) Some scientists call EGFR Tyr869 as EGFR Tyr845, the site where the signaling peptide is removed.