CoraLite® Plus 647-conjugated Phospho-JNK (Tyr185) Recombinant monoclonal antibody

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• CoraLite® Plus 647-conjugated Phospho-JNK (Tyr185) Recombinant monoclonal antibody (CL647-80024)

Phospho-JNK (Tyr185) Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL647-80024

Clone No.2G8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Synonyms

JNK (Tyr185), p JNK, p JNK (Tyr185), p JNK Tyr185, Phospho JNK

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  FC experiment of HEK-293 using CL647-80024

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.2 ug Coralite® Plus 647 Phospho-JNK (Tyr185) Recombinant Antibody (CL647-80024, Clone:2G8)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: Calyculin A treated HEK-293 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-80024 targets Phospho-JNK (Tyr185) in FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mitogen-activated protein kinase 8
• Observed Molecular Weight: 37-45kDa
• GenBank Accession Number: NM_139049
• Gene Symbol: JNK
• Gene ID (NCBI): 5599
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P45983
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun.