Phospho-SH3PXD2B (Ser291) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84563-1-RR (same clone as 84563-1-PBS)

  Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 84563-1-RR (Phospho-SH3PXD2B (Ser291) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84563-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84563-1-RR (same clone as 84563-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated HeLa cells using Phospho-SH3PXD2B (Ser291) antibody (84563-1-RR, Clone: 241064C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84563-1-PBS in a different storage buffer formulation.

Phospho-SH3PXD2B (Ser291) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 84563-1-PBS

Clone No.241064C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Phospho-SH3PXD2B, Phospho-SH3PXD2B Ser291, P-SH3PXD2B, P-SH3PXD2B (Ser291), SH3PXD2B (Ser291)

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Product Information

84563-1-PBS targets Phospho-SH3PXD2B (Ser291) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: SH3 and PX domains 2B
• Calculated Molecular Weight: 911aa, 102 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: NM_001017995
• Gene Symbol: SH3PXD2B
• Gene ID (NCBI): 285590
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: A1X283
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SH3 and PX domains 2B (SH3PXD2B) is also known as tyrosine kinase substrate with four SH3 domains (Tks4). It consists of the N-terminal phox homology domain, four Sr homology 3 domains, several proline-rich regions, and Src phosphorylation sites. Previous studies have shown that SH3PXD2B is an essential invadopodium scaffold protein and can stimulate actin polymerization, recruit membrane type-1 matrix metalloproteinase (MT1-MMP) to intracellular membranes, and increase Nox1-dependent reactive oxygen species (ROS) production. (PMID: 33906640)