Phospho-SH3PXD2B (Ser291) Recombinant antibody

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  WB analysis using 84563-1-RR

  Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 84563-1-RR (Phospho-SH3PXD2B (Ser291) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

Phospho-SH3PXD2B (Ser291) Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84563-1-RR

Clone No.241064C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Phospho-SH3PXD2B, Phospho-SH3PXD2B Ser291, P-SH3PXD2B, P-SH3PXD2B (Ser291), SH3PXD2B (Ser291)

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Tested Applications

• Positive WB detected in: HeLa cells, λ phosphatase treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84563-1-RR targets Phospho-SH3PXD2B (Ser291) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: SH3 and PX domains 2B
• Calculated Molecular Weight: 911aa, 102 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: NM_001017995
• Gene Symbol: SH3PXD2B
• Gene ID (NCBI): 285590
• RRID: AB_3672071
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: A1X283
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SH3 and PX domains 2B (SH3PXD2B) is also known as tyrosine kinase substrate with four SH3 domains (Tks4). It consists of the N-terminal phox homology domain, four Sr homology 3 domains, several proline-rich regions, and Src phosphorylation sites. Previous studies have shown that SH3PXD2B is an essential invadopodium scaffold protein and can stimulate actin polymerization, recruit membrane type-1 matrix metalloproteinase (MT1-MMP) to intracellular membranes, and increase Nox1-dependent reactive oxygen species (ROS) production. (PMID: 33906640)