CoraLite® Plus 647-conjugated Phospho-eEF2 (Thr57) Recombinant monoclonal antibody

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• CoraLite® Plus 647-conjugated Phospho-eEF2 (Thr57) Recombinant monoclonal antibody (CL647-80351-2)

Phospho-eEF2 (Thr57) Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL647-80351-2 Copied!

Clone No. 250222A6

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

p eEF2, p eEF2 (Thr57), p-eEF2, p-eEF2 (Thr57), p-eEF2(Thr57)

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  FC experiment of HeLa using CL647-80351-2

  1X10^6 HeLa cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-Eef2 (Thr57) Recombinant Antibody (CL647-80351-2, Clone:250222A6)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: λ phosphatase treated HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-80351-2 targets Phospho-eEF2 (Thr57) in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: eukaryotic translation elongation factor 2
• Calculated Molecular Weight: 858 aa, 95 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC126259
• Gene Symbol: EEF2
• Gene ID (NCBI): 1938
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13639
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

eEF2 (Eukaryotic Elongation Factor 2) is a key component in the process of protein synthesis in eukaryotic cells. It is a GTP-binding protein that plays a crucial role in the elongation phase of translation. Specifically, eEF2 facilitates the movement of the ribosome along the mRNA, allowing the addition of amino acids to the growing polypeptide chain. This elongation factor is essential for efficient and accurate protein synthesis. Interestingly, eEF2 phosphorylation can either be increased or decreased depending on the stimulus used in learning or synaptic plasticity paradigms. Examining the specific contributions of increases and decreases in eEF2 phosphorylation to learning and memory is difficult. (PMID: 25762683)