|Positive WB detected in||HeLa cells, NIH/3T3 cells, HEK-293T cells, HEK-293 cells, HSC-T6 cells|
|Positive IHC detected in||human colon cancer tissue, human urothelial carcinoma tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
67778-1-Ig targets Phospho-mTOR (Ser2448) in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse samples.
|Tested Reactivity||Human, Mouse|
|Cited Reactivity||bovine, human, pig, rat|
|Host / Isotype||Mouse / IgG2b|
|Full Name||FK506 binding protein 12-rapamycin associated protein 1|
|Calculated molecular weight||289 kDa|
|Observed molecular weight||289 kDa|
|GenBank accession number||BC117166|
|Gene ID (NCBI)||2475|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.
|Product Specific Protocols|
|WB protocol for Phospho-mTOR (Ser2448) antibody 67778-1-Ig||Download protocol|
|IHC protocol for Phospho-mTOR (Ser2448) antibody 67778-1-Ig||Download protocol|
|IF protocol for Phospho-mTOR (Ser2448) antibody 67778-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Lilas (Verified Customer) (06-29-2021)
Used antibody at a 1:1000 dilution in 5% BSA, and overnight incubation at 4 degrees. A sharp band detected above the 245 kDA marker using western blot.