CoraLite®488-conjugated Phospho-mTOR (Ser2448) Monoclonal antibody

Phospho-mTOR (Ser2448) Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

2A12G3

Cat no : CL488-67778

Synonyms

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67778-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-67778 targets Phospho-mTOR (Ser2448) in IF applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name FK506 binding protein 12-rapamycin associated protein 1
Calculated molecular weight 289 kDa
GenBank accession numberBC117166
Gene symbol FRAP1
Gene ID (NCBI) 2475
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.

Protocols

Product Specific Protocols
IF protocol for CL488 Phospho-mTOR (Ser2448) antibody CL488-67778Download protocol
Standard Protocols
Click here to view our Standard Protocols