CoraLite® Plus 488-conjugated RAB11FIP2 Polyclonal antibody

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  IF Staining of HeLa using CL488-18136

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 RAB11FIP2 antibody (CL488-18136) at dilution of 1:200, CL594-Phalloidin (red).

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  IF Staining of HepG2 using CL488-18136

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 RAB11FIP2 antibody (CL488-18136) at dilution of 1:200.

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  FC experiment of HeLa using CL488-18136

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated RAB11FIP2 Polyclonal antibody (CL488-18136)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

RAB11FIP2 Polyclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-18136

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

NRip11, Rab11 family-interacting protein 2, Rab11 FIP2, Rab11-FIP2

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-18136 targets RAB11FIP2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12736

  Product name: Recombinant human RAB11FIP2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-373 aa of BC075073

  Sequence: MMLSEQAQKWFPTHVQVTVLQAKDLKPKGKSGTNDTYTIIQLGKEKYSTSVAEKTLEPVWKEEASFELPGLLIQGSPEKYILFLIVMHRSLVGLDKFLGQVAINLNDIFEDKQRRKTEWFRLESKQGKRIKNRGEIKVNIQFMRNNMTASMFDLSMKDKTRSPFAKLKDKMKGRKNDGTFSDTSSAIIPSTHMPDANSEFSSGEIQMKSKPKKPFLLGPQRLSSAHSMSDLSGSHMSSEKLKAGTIGQTHLLGHQLDSFGTVPESGSLKSPHRRTLSFDTSKMNQPDSIVDEGELCFGRQNDPFTNVTASLPQKFATLPRKKNPFEESSETWDSSMNLFSKPIEIRKENKREKREKVSLFERVTGKKDSRRSD

• Full Name: RAB11 family interacting protein 2 (class I)
• Calculated Molecular Weight: 512 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC075073
• Gene Symbol: RAB11FIP2
• Gene ID (NCBI): 22841
• RRID: AB_3672688
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7L804
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RAB11FIP2, or RAB11 family interacting protein 2, is a protein that plays a crucial role in intracellular trafficking, particularly in the recycling of endocytic vesicles. It is involved in several cellular processes, including TRAM-dependent toll-like receptor 4 signaling pathway, phagocytosis, and positive regulation of GTPase activity. RAB11FIP2 is localized in endosomes, nucleoplasm, and phagocytic cups, and it is expressed ubiquitously in various tissues, with higher expression in endometrium and brain. RAB11FIP2 interacts with the actin-based motor myo5b and the small GTPase Rab11, which are key components in the regulation of vesicle tethering and transport along actin filaments. This interaction is particularly important for processes such as cell polarization, motility, and lumen formation in multicellular structures. .