RAB13 Polyclonal antibody, PBS Only

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• RAB13 Polyclonal antibody, PBS Only (31416-1-PBS)

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  WB analysis using 31416-1-AP (same clone as 31416-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31416-1-AP (RAB13 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31416-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 31416-1-AP (same clone as 31416-1-PBS)

  WB result of RAB13 antibody (31416-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB13 transfected HEK-293 cells. This data was developed using the same antibody clone with 31416-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 31416-1-AP (same clone as 31416-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31416-1-AP (RAB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31416-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 31416-1-AP (same clone as 31416-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31416-1-AP (RAB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31416-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 31416-1-AP (same clone as 31416-1-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.8 ug RAB13 Polyclonal antibody (31416-1-AP) and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 31416-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RAB13 Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 31416-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

GIG4, Cell growth inhibiting gene 4 protein, Cell growth-inhibiting gene 4 protein, Ras related protein Rab 13, Ras-related protein Rab-13

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Product Information

31416-1-PBS targets RAB13 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34898

  Product name: Recombinant human RAB13 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 100-200 aa of BC000799

  Sequence: QNWMKSIKENASAGVERLLLGNKCDMEAKRKVQKEQADKLAREHGIRFFETSAKSSMNVDEAFSSLARDILLKSGGRRSGNGNKPPSTDLKTCDKKNTNKC

• Full Name: RAB13, member RAS oncogene family
• Calculated Molecular Weight: 203 aa, 23 kDa
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: BC000799
• Gene Symbol: RAB13
• Gene ID (NCBI): 5872
• RRID: AB_3669972
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P51153
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).