RAB26 Polyclonal antibody

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• RAB26 Polyclonal antibody (14284-1-AP)

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• KD/KO Validated

RAB26 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14284-1-AP Copied!

Publications (13)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Ras related protein Rab 26, Ras-related protein Rab-26

• 

  WB analysis of mouse pancreas using 14284-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14284-1-AP (RAB26 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse pancreas using 14284-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14284-1-AP (RAB26 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse pancreas using 14284-1-AP

  IP result of anti-RAB26 (IP:14284-1-AP, 4ug; Detection:14284-1-AP 1:600) with mouse pancreas tissue lysate 2480 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 14284-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14284-1-AP (RAB26 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 14284-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14284-1-AP (RAB26 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 14284-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RAB26 antibody (14284-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of BxPC-3 using 14284-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed BxPC-3 cells using 14284-1-AP (RAB26 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of MCF-7 using 14284-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human RAB26 (14284-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse pancreas tissue
• Positive IP detected in: mouse pancreas tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells, BxPC-3 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14284-1-AP targets RAB26 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5581

  Product name: Recombinant human RAB26 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-256 aa of BC066913

  Sequence: MSRKKTPKSKGASTPAASTLPTANGARPARSGTALSGPDAPPNGPLQPGRPSLGGGVDFYDVAFKVMLVGDSGVGKTCLLVRFKDGAFLAGTFISTVGIDFRNKVLDVDGVKVKLQMWDTAGQERFRSVTHAYYRDAHALLLLYDVTNKASFDNIQAWLTEIHEYAQHDVALMLLGNKVDSAHERVVKREDGEKLAKEYGLPFMETSAKTGLNVDLAFTAIAKELKRRSMKAPSEPRFRLHDYVKREGRGASCCRP

• Full Name: RAB26, member RAS oncogene family
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC066913
• Gene Symbol: RAB26
• Gene ID (NCBI): 25837
• RRID: AB_2176709
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9ULW5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RAB26 Polyclonal antibody (Cat# 14284-1-AP) has 13 total citations. These appear in journals including Genes Discovery, Cell Death & Disease, Science of the Total Environment, Free Radical Biology and Medicine, PLoS Biology, Cell & Molecular Life Sciences, Life Sciences, Cancers, iScience, Journal of Cell Science, and Journal of Immunology Research. Research fields span oncology (prostate, breast, and lung cancer), neurology and synaptic toxicity, COPD/airway inflammation, insulin secretion, cardiac remodeling, and lysosomal/autophagy trafficking.

Automatically generated from the 13 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Genes Dis; RAB26 promotes prostate cancer progression via the MAPK/ERK-TWIST1 signaling axis; Authors - Hexi Wang
human	WB,IHC	Cell Death Dis; Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src.; Authors - Huiying Liu
human	IHC	Cell Death Dis; SNRPB promotes the tumorigenic potential of NSCLC in part by regulating RAB26.; Authors - Nianli Liu ; KO Validated
mouse	WB	Sci Total Environ; Manganese disrupts the maturation and degradation of axonal autophagosome leading to hippocampal synaptic toxicity in mice via the activation of LRRK2 on phosphorylation of Rab10; Authors - Zhuo Ma
mouse,human	WB,IHC	Free Radic Biol Med; ROS induced the Rab26 promoter hypermethylation to promote cigarette smoking-induced airway epithelial inflammation of COPD through activation of MAPK signaling; Authors - Bin-Feng He ; KD Validated
mouse	WB,IP,IF	PLoS Biol; Rab26 restricts insulin secretion via sequestering Synaptotagmin-1; Authors - Ruijuan Zhuang ; KO Validated