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- KD/KO Validated
RAB27A Monoclonal antibody
RAB27A Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
WB, IHC, ELISA
Cat no : 66058-1-Ig
Validation Data Gallery
|Positive WB detected in
|HeLa cells, K562, K-562 cells, Jurkat cells, HEK-293 cells, HL-60 cells, HepG2 cells
|Positive IHC detected in
|human lymphoma tissue, human colon cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:5000-1:50000
|IHC : 1:5000-1:20000
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66058-1-Ig targets RAB27A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Host / Isotype
|Mouse / IgG1
|RAB27A fusion protein Ag12382
|RAB27A, member RAS oncogene family
|Calculated molecular weight
|221 aa, 25 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.