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RAB27A Monoclonal antibody

RAB27A Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1C4B8

Cat no : 66058-1-Ig

Synonyms

GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A



Tested Applications

Positive WB detected inHeLa cells, K-562 cells, Jurkat cells, HEK-293 cells, HL-60 cells, HepG2 cells
Positive IHC detected inhuman lymphoma tissue, human colon cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66058-1-Ig is RAB27A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAB27A fusion protein Ag12382
Full Name RAB27A, member RAS oncogene family
Calculated molecular weight 221 aa, 25 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC107680
Gene symbol RAB27A
Gene ID (NCBI) 5873
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab27a is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27a is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27a is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.

Protocols

Product Specific Protocols
WB protocol for RAB27A antibody 66058-1-IgDownload protocol
IHC protocol for RAB27A antibody 66058-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols