CoraLite®594-conjugated RAB3GAP1 Monoclonal antibody

RAB3GAP1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4A3A1

Cat no : CL594-66688

Synonyms

DKFZp434A012, KIAA0066, P130, Rab3 GAP, Rab3 GAP p130, RAB3GAP, RAB3GAP1, RAB3GAP130, WARBM1

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Validation Data Gallery

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  IF Staining of HEK-293 using CL594-66688

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®594 RAB3GAP1 antibody (CL594-66688, Clone: 4A3A1 ) at dilution of 1:100.

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  FC experiment of HEK-293 using CL594-66688

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RAB3GAP1 (CL594-66688, Clone:4A3A1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HEK-293 cells
• Positive FC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66688 targets RAB3GAP1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB3GAP1 fusion protein Ag17710
• Full Name: RAB3 GTPase activating protein subunit 1 (catalytic)
• Calculated Molecular Weight: 981 aa, 111 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC146809
• Gene Symbol: RAB3GAP1
• Gene ID (NCBI): 22930
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.