RABEP2 Monoclonal antibody

• Featured Product
• KD/KO Validated

RABEP2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A4A4

Cat no : 60125-1-Ig

Synonyms

FRA, Rabaptin 5beta, RABEP2, RABPT5B

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Validation Data Gallery

• 

  WB analysis of Jurkat using 60125-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 60125-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 60125-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 60125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60125-1-Ig (RABEP2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60125-1-Ig (RABEP2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60125-1-Ig (RABEP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60125-1-Ig (RABEP2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HSC-T6 cells, Jurkat cells, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60125-1-Ig targets RABEP2 in WB, IHC, ELISA applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RABEP2 fusion protein Ag6197
• Full Name: rabaptin, RAB GTPase binding effector protein 2
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC058900
• Gene Symbol: RABEP2
• Gene ID (NCBI): 79874
• RRID: AB_2284821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RABEP2, also named as RABPT5B, plays a role in membrane trafficking and in homotypic early endosome fusion. It has two isoforms with MW 59-63 kDa.