RAD21 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAD21 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 27071-1-AP

Synonyms

hHR21, HR21, HRAD21, KIAA0078, MCD1, Nuclear matrix protein 1, NXP 1, NXP1, RAD21, RAD21 homolog (S. pombe), SCC1, SCC1 homolog

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Validation Data Gallery

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  WB analysis of A549 using 27071-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27071-1-AP (RAD21 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27071-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27071-1-AP (RAD21 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27071-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27071-1-AP (RAD21 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27071-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27071-1-AP (RAD21 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MDCK using 27071-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using RAD21 antibody (27071-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of MDCK using 27071-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using RAD21 antibody (27071-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

27071-1-AP targets RAD21 in WB, IF, IHC, ELISA applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAD21 fusion protein Ag25481
• Full Name: RAD21 homolog (S. pombe)
• Calculated Molecular Weight: 631 aa, 72 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC050381
• Gene Symbol: RAD21
• Gene ID (NCBI): 5885
• RRID: AB_2880742
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAD21, also named as Nuclear matrix protein 1, is a 631 amino acid protein, which belongs to the rad21 family. The calculated molecular weight of RAD21 is 72 kDa, but the phosphorylated RAD21 is about 120 kDa. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells

Publications

Species	Application	Title
human	WB	Cancer Lett; IQGAP3 interacts with Rad17 to recruit the Mre11-Rad50-Nbs1 complex and contributes to radioresistance in lung cancer.; Authors - Yulan Zeng
human	IHC,WB	Front Oncol; RAD21 Confers Poor Prognosis and Affects Ovarian Cancer Sensitivity to Poly(ADP-Ribose)Polymerase Inhibitors Through DNA Damage Repair.; Authors - Rui Gou ; KD Validated
human	WB	Cell Death Dis; EGFR-ERK induced activation of GRHL1 promotes cell cycle progression by up-regulating cell cycle related genes in lung cancer.; Authors - Yiming He