MOK Polyclonal antibody

• Featured Product
• KD/KO Validated

MOK Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 23926-1-AP

Synonyms

MOK, MOK protein kinase, RAGE, RAGE 1, RAGE1, renal tumor antigen, Renal tumor antigen 1

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Validation Data Gallery

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  WB analysis of HeLa using 23926-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23926-1-AP (MOK antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 23926-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23926-1-AP (MOK antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23926-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23926-1-AP (MOK antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 23926-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 23926-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 23926-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 23926-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23926-1-AP (MOK antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 23926-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23926-1-AP (MOK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 23926-1-AP

  1X10^6 HeLa cells were stained with 0.2ug MOK antibody (23926-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human hepatocirrhosis tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

The immunogen of 23926-1-AP is MOK Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MOK fusion protein Ag20849
• Full Name: renal tumor antigen
• Calculated Molecular Weight: 231 aa, 27 kDa
• Observed Molecular Weight: 42 kDa, 85 kDa
• GenBank Accession Number: BC053536
• Gene Symbol: RAGE
• Gene ID (NCBI): 5891
• RRID: AB_2879364
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Oncol; Proteomics and phosphoproteomics of chordoma biopsies reveal alterations in multiple pathways and aberrant kinases activities; Authors - Jing Hang ; KD Validated
	WB	J Biol Chem; Serine/Threonine Kinase Unc-51-like Kinase-1 (Ulk1) Phosphorylates the Co-chaperone Cell Division Cycle Protein 37 (Cdc37) and Thereby Disrupts the Stability of Cdc37 Client Proteins.; Authors - Ran Li