Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 RALA antibody (CL594-67093, Clone: 1H9H5 ) at dilution of 1:200.
FC experiment of MCF-7 using CL594-67093
1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human RALA (CL594-67093, Clone:1H9H5) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human RALA (CL594-67093, Clone:1H9H5) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
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Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 RALA antibody (CL594-67093, Clone: 1H9H5 ) at dilution of 1:200.
FC (INTRA) Figures
FC experiment of MCF-7 using CL594-67093
1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human RALA (CL594-67093, Clone:1H9H5) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
RALA Monoclonal antibody
Catalog Number
CL594-67093
Citations
-
Dilutions
IF/ICC : 1:50-1:500 FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Applications
IF/ICC, FC (Intra)
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
