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  • KD/KO Validated

RAN Polyclonal antibody

RAN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10469-1-AP

Synonyms

ARA24, Androgen receptor-associated protein 24, GTP-binding nuclear protein Ran, GTPase Ran, GTP binding nuclear protein Ran



Tested Applications

Positive WB detected inHEK-293 cells, A375 cells, rat kidney tissue, NIH/3T3 cells, mouse kidney tissue, HeLa cells, Jurkat cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman breast cancer tissue, human colon tissue, human testis tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10469-1-AP targets RAN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, macrobrachium rosenbergii
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAN fusion protein Ag0748
Full Name RAN, member RAS oncogene family
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC004272
Gene Symbol RAN
Gene ID (NCBI) 5901
RRIDAB_2176484
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RAN antibody 10469-1-APDownload protocol
IHC protocol for RAN antibody 10469-1-APDownload protocol
IF protocol for RAN antibody 10469-1-APDownload protocol
IP protocol for RAN antibody 10469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,IP

Cell Death Dis

RSL1D1 promotes the progression of colorectal cancer through RAN-mediated autophagy suppression.

Authors - Xunhua Liu
  • KD Validated
human,ratWB

Diabetologia

Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.

Authors - Mu Zhang
ratIP,WB

Cell Commun Signal

Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression.

Authors - Abiola Abdulrahman Ayanlaja
humanIHC

Oncotarget

Identification of nasopharyngeal carcinoma metastasis-related biomarkers by iTRAQ combined with 2D-LC-MS/MS.

Authors - Zhen Chen
humanWB

Int J Biol Sci

Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression.

Authors - Yancheng Zhong
  • KD Validated
humanIF

J Cell Sci

A nuclear export sequence promotes CRM1-dependent targeting of the nucleoporin Nup214 to the nuclear pore complex.

Authors - Mohamed Hamed

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

María (Verified Customer) (02-08-2022)

Works very well for WB (1:1000, 1h) and IF in permeabilized cells (1:200). Validated with siRNA

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: RPE-1/MSC