- Featured Product
- KD/KO Validated
RAN Polyclonal antibody
RAN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 10469-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, A375 cells, rat kidney tissue, NIH/3T3 cells, mouse kidney tissue, HeLa cells, Jurkat cells |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | human breast cancer tissue, human colon tissue, human testis tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 10 publications below |
IHC | See 2 publications below |
IF | See 3 publications below |
IP | See 3 publications below |
Product Information
10469-1-AP targets RAN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, macrobrachium rosenbergii |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RAN fusion protein Ag0748 |
Full Name | RAN, member RAS oncogene family |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 24 kDa |
GenBank Accession Number | BC004272 |
Gene Symbol | RAN |
Gene ID (NCBI) | 5901 |
RRID | AB_2176484 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for RAN antibody 10469-1-AP | Download protocol |
IHC protocol for RAN antibody 10469-1-AP | Download protocol |
IF protocol for RAN antibody 10469-1-AP | Download protocol |
IP protocol for RAN antibody 10469-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Dis RSL1D1 promotes the progression of colorectal cancer through RAN-mediated autophagy suppression.
| ||
Diabetologia Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function. | ||
Cell Commun Signal Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression. | ||
Oncotarget Identification of nasopharyngeal carcinoma metastasis-related biomarkers by iTRAQ combined with 2D-LC-MS/MS. | ||
Int J Biol Sci Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression.
| ||
J Cell Sci A nuclear export sequence promotes CRM1-dependent targeting of the nucleoporin Nup214 to the nuclear pore complex. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH María (Verified Customer) (02-08-2022) | Works very well for WB (1:1000, 1h) and IF in permeabilized cells (1:200). Validated with siRNA
|