|Positive WB detected in||HEK-293 cells, A375 cells, rat kidney tissue, NIH/3T3 cells, mouse kidney tissue, HeLa cells, Jurkat cells|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human breast cancer tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10469-1-AP targets RAN in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, Macrobrachium Rosenbergii, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RAN fusion protein Ag0748|
|Full Name||RAN, member RAS oncogene family|
|Calculated molecular weight||25 kDa|
|Observed molecular weight||24 kDa|
|GenBank accession number||BC004272|
|Gene ID (NCBI)||5901|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
|Product Specific Protocols|
|WB protocol for RAN antibody 10469-1-AP||Download protocol|
|IHC protocol for RAN antibody 10469-1-AP||Download protocol|
|IF protocol for RAN antibody 10469-1-AP||Download protocol|
|IP protocol for RAN antibody 10469-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochim Biophys Acta Mol Cell Res
SIRT7 regulates the nuclear export of NF-κB p65 by deacetylating Ran.
Fish Shellfish Immunol
Identification and function analysis of ras-related nuclear protein from Macrobrachium rosenbergii involved in Spiroplasma eriocheiris infection.
J Cell Sci
A nuclear export sequence promotes CRM1-dependent targeting of the nucleoporin Nup214 to the nuclear pore complex.
Int J Biol Sci
Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression.
Identification of nasopharyngeal carcinoma metastasis-related biomarkers by iTRAQ combined with 2D-LC-MS/MS.
Cell Commun Signal
Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression.