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RAPGEF5 Polyclonal antibody, PBS Only (12556-1-PBS)

Filter: AllWBIP

WB analysis using 12556-1-AP (same clone as 12556-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12556-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 12556-1-AP (same clone as 12556-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12556-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 12556-1-AP (same clone as 12556-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12556-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 12556-1-AP (same clone as 12556-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12556-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 12556-1-AP (same clone as 12556-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12556-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 12556-1-AP (same clone as 12556-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12556-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 12556-1-AP (same clone as 12556-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12556-1-PBS in a different storage buffer formulation.
IP experiment of K-562 using 12556-1-AP (same clone as 12556-1-PBS)
IP result of anti-RAPGEF5 (IP:12556-1-AP, 4ug; Detection:12556-1-AP 1:500) with K-562 cells lysate 1760 ug. This data was developed using the same antibody clone with 12556-1-PBS in a different storage buffer formulation.

RAPGEF5 Polyclonal antibody, PBS Only

Name: RAPGEF5 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 12556-1-PBS

RAPGEF5 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 12556-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Synonyms

M-Ras-regulated Rap GEF, Rap guanine nucleotide exchange factor 5

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

12556-1-PBS targets RAPGEF5 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3229 Product name: Recombinant human RAPGEF5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 129-444 aa of BC039203 Sequence: KKYQGKEENSDVPRRKRKVLHLVSQWIALYKDWLPEDEHSKMFLKTIYRNVLDDVYEYPILEKELKEFQKILGMHRRHTVDEYSPQKKNKALFHQFSLKENWLQHRGTVTETEEIFCHVYITEHSYVSVKAKVSSIAQEILLCSQLGKRVQLVKKFIKIAAHCKAQRNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKMKPPKIPFVPLLLKDVTFIHEGNKTFLDNLVNFEKLHMIADTVRTLRHCRTNQFGDLSPKEHQELKSYVNHLYVIDSQQALFELSHRIEPRV Predict reactive species
Full Name	Rap guanine nucleotide exchange factor (GEF) 5
Calculated Molecular Weight	68 kDa
Observed Molecular Weight	68-75 kDa
GenBank Accession Number	BC039203
Gene Symbol	RAPGEF5
Gene ID (NCBI)	9771
RRID	AB_2177476
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92565
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.