RASAL2 Polyclonal antibody, PBS Only

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• RASAL2 Polyclonal antibody, PBS Only (22140-1-PBS)

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• KD/KO Validated

RASAL2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 22140-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Ras GTPase activating protein nGAP, Ras GTPase-activating protein nGAP, RAS protein activator like 2, RAS protein activator-like 2, RASAL 2

• 

  WB analysis of HeLa using 22140-1-AP (same clone as 22140-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22140-1-AP (RASAL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22140-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 22140-1-AP (same clone as 22140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22140-1-AP (RASAL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22140-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat testis using 22140-1-AP (same clone as 22140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22140-1-AP (RASAL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22140-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 22140-1-AP (same clone as 22140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22140-1-AP (RASAL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22140-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 22140-1-AP (same clone as 22140-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22140-1-AP (RASAL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22140-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

22140-1-PBS targets RASAL2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17555

  Product name: Recombinant human RASAL2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 609-958 aa of BC110611

  Sequence: LRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDERESSLPNGRSVSLMDLQDTHAAQVEHASVMLDVPIRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGGLQPLSFQNPVYHLNNPIPAMPKASIDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFT

• Full Name: RAS protein activator like 2
• Calculated Molecular Weight: 1262 aa, 142 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC110611
• Gene Symbol: RASAL2
• Gene ID (NCBI): 9462
• RRID: AB_2879005
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJF2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RASAL2 (RAS protein activator like 2) is a protein-coding gene that encodes a member of the RAS GTPase-activating protein (RAS-GAP) family. The encoded protein contains a characteristic GAP-related domain (GRD) that enables it to catalyze the hydrolysis of RAS-GTP to RAS-GDP, thereby functioning as a negative regulator of RAS signaling. Structurally, RASAL2 also possesses additional domains including a Pleckstrin homology (PH) domain and a C2 domain, which are involved in membrane localization and calcium/lipid binding. RASAL2 has been extensively studied for its context-dependent roles in cancer, predominantly functioning as a tumor suppressor in malignancies such as breast, bladder, renal cell, ovarian, and lung cancers, where its downregulation or loss promotes tumor growth, stemness, epithelial-mesenchymal transition (EMT), and metastasis.