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CoraLite® Plus 488-conjugated RBM15 Monoclonal antibody
RBM15 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4A1A4
Cat no : CL488-66059
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of CL488-66059 is RBM15 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | RBM15 fusion protein Ag17970 |
Full Name | RNA binding motif protein 15 |
Calculated Molecular Weight | 105 kDa |
Observed Molecular Weight | 107 kDa |
GenBank Accession Number | BC006397 |
Gene Symbol | RBM15 |
Gene ID (NCBI) | 64783 |
RRID | AB_2919282 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 488 RBM15 antibody CL488-66059 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |