RBM15B Monoclonal antibody

RBM15B Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


Human, rat and More (1)







Cat no : 67506-1-Ig


HUMAGCGB, HuOTT3, One twenty two protein 3, OTT3, RBM15B, RNA binding motif protein 15B

Tested Applications

Positive WB detected inHeLa cells, A549 cells, K-562 cells, COLO 320 cells, LNCaP cells, HEK-293 cells, Jurkat cells, THP-1 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

67506-1-Ig targets RBM15B in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RBM15B fusion protein Ag17853
Full Name RNA binding motif protein 15B
Calculated molecular weight 890 aa, 97 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC001367
Gene symbol RBM15B
Gene ID (NCBI) 29890
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM15B, one members of the SPEN (Split-end) family of proteins, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components. Present polyclonal anti-RBM15B antibody was produced by immunizing animals with C-terminus of RBM15B, which homolog with a short form of RBM15B(563aa, ~70kDa).


Product Specific Protocols
WB protocol for RBM15B antibody 67506-1-IgDownload protocol
IHC protocol for RBM15B antibody 67506-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Biol Chem

Wilms' Tumor 1-Associating Protein complex regulates alternative splicing and polyadenylation at potential G-quadruplex-forming splice site sequences.

Authors - Keiko Horiuchi

Nat Commun

CSTF2 mediated mRNA N6-methyladenosine modification drives pancreatic ductal adenocarcinoma m6A subtypes

Authors - Yanfen Zheng