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CoraLite® Plus 488-conjugated RBP5 Monoclonal antibody

RBP5 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G7E2

Cat no : CL488-68126

Synonyms

CRBP III, CRBP3, CRBPIII, HRBPiso, RBP5, Retinol binding protein 5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated RBP5 Monoclonal anti (CL488-68126)

    IF Staining of HepG2 using CL488-68126

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 RBP5 antibody (CL488-68126, Clone: 1G7E2 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated RBP5 Monoclonal anti (CL488-68126)

    IF Staining of A431 using CL488-68126

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 RBP5 antibody (CL488-68126, Clone: 1G7E2 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHepG2 cells, A431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68126 targets RBP5 in IF applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RBP5 fusion protein Ag24562
Full Name retinol binding protein 5, cellular
Calculated Molecular Weight 135 aa, 16 kDa
Observed Molecular Weight 16-18 kDa
GenBank Accession NumberBC029355
Gene Symbol RBP5
Gene ID (NCBI) 83758
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBP5 (CRBP III) belongs to the fatty-acid binding protein (FABP) family. Human liver and kidney contain the highest levels of CRBP III mRNA, and CRBP III is as a major human intracellular carrier of retinol (PMID:11274389). RBP5 iron response are reversible by genetic complementation. And increased RBP5 expression occurs by a post-transcriptional feedback mechanism whereby RBP5 interacts with its own, and with PAP2 mRNAs. RBP5 is the most significant of the proteins upregulated by iron starvation (PMID:34161395). RBP5 has an estimated molecular mass (15.9 kDa) typical of this protein superfamily (PMID:11274389).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RBP5 antibody CL488-68126Download protocol
Standard Protocols
Click here to view our Standard Protocols