RCC1 Monoclonal antibody

RCC1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C2F5

Cat no : 67335-1-Ig

Synonyms

Cell cycle regulatory protein, CHC1, RCC1, RCC1 I

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Validation Data Gallery

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  WB analysis using 67335-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67335-1-Ig (RCC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67335-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 67335-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 67335-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67335-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: mouse brain tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67335-1-Ig targets RCC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RCC1 fusion protein Ag17809
• Full Name: regulator of chromosome condensation 1
• Calculated Molecular Weight: 421 aa, 45 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC010067
• Gene Symbol: RCC1
• Gene ID (NCBI): 1104
• RRID: AB_2882593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation.