CoraLite® Plus 488-conjugated RNF31 / HOIP Recombinant monoclonal antibody

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  IF Staining of HepG2 using CL488-83137-3

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 RNF31 / HOIP antibody (CL488-83137-3, Clone: 230446H1 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-83137-3

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Rnf31 / HOIP Recombinant Antibody (CL488-83137-3, Clone:230446H1)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

RNF31 / HOIP Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra)

Cat No. CL488-83137-3

Clone No.230446H1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

HOIP, RNF31, HOIL 1 interacting protein, FLJ23501, FLJ10111

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83137-3 targets RNF31 / HOIP in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag34505

  Product name: Recombinant human RNF31 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 459-603 aa of BC017376

  Sequence: RDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQSIPRRRK

• Full Name: ring finger protein 31
• Calculated Molecular Weight: 1072 aa, 120 kDa
• GenBank Accession Number: BC017376
• Gene Symbol: RNF31
• Gene ID (NCBI): 55072
• RRID: AB_3673200
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96EP0
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.