CoraLite® Plus 488-conjugated RNMTL1 Polyclonal antibody

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  IF Staining of HeLa using CL488-14707

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 RNMTL1 antibody (CL488-14707) at dilution of 1:200, CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

RNMTL1 Polyclonal Antibody for IF/ICC

Cat No. CL488-14707

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

FLJ10581, HC90, MRM3, RNA methyltransferase like 1, RNMTL1

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14707 targets RNMTL1 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6399

  Product name: Recombinant human RNMTL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-420 aa of BC050614

  Sequence: MAALVRPSRFVVRPLLQVVQAWDLDARRWVRALRRSPVKVVFPSGEVVEQKRAPGKQPRKAPSEASAQEQREKQPLEESASRAPSTWEESGLRYDKAYPGDRRLSSVMTIVKSRPFREKQGKILLEGRRLISDALKAGAVPKMFFFSRLEYLKELPVDKLKGVSLIKVKFEDIKDWSDLVTPQGVMGIFAKPDHVKMTYPKTQLQHSLPLLLICDNLRDPGNLGTILRSAAGAGCSKVLLTKGCVDAWEPKVLRAGMGAHFRMPIINNLEWETVPNYLPPDTRVYVADNCGLYAQAEMSNKASDHGWVCDQRVMKFHKYEEEEDVETGASQDWLPHVEVQSYDSDWTEAPAAVVIGGETYGVSLESLQLAESTGGKRLLIPVVPGVDSLNSAMAASILLFEGKRQLRGRAEDLSRDRSYH

• Full Name: RNA methyltransferase like 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC050614
• Gene Symbol: RNMTL1
• Gene ID (NCBI): 55178
• RRID: AB_3672600
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HC36
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The RNA methyltransferase like 1 gene (RNMTL1) is one of thirteen newly discovered genes within a 116 Kb segment of the chromosome 17p13.3, which suffers from a high frequent loss of heterozygosity in human hepatocellular carcinoma. Its amino acid sequence was identified a high conserved region with methylases from various microorganisms, suggesting to have function to transfer methyl group to rRNA molecule [PMID:12296377].