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  • KD/KO Validated

ROCK1 Polyclonal antibody

ROCK1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21850-1-AP

Synonyms

p160 ROCK 1, P160ROCK, PRO0435, ROCK1



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21850-1-AP is ROCK1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ROCK1 fusion protein Ag16317
Full Name Rho-associated, coiled-coil containing protein kinase 1
Calculated molecular weight 1354 aa, 158 kDa
Observed molecular weight 150-160 kDa
GenBank accession numberBC113114
Gene symbol ROCK1
Gene ID (NCBI) 6093
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.

Protocols

Product Specific Protocols
WB protocol for ROCK1 antibody 21850-1-APDownload protocol
IHC protocol for ROCK1 antibody 21850-1-APDownload protocol
IF protocol for ROCK1 antibody 21850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Biochem

Chondroitin sulfate proteoglycan represses neural stem/progenitor cells migration via PTPσ/α-actinin4 signaling pathway.

Authors - Jun Zhong
humanWB,IF

Acta Biomater

ROCK isoforms differentially modulate cancer cell motility by mechanosensing the substrate stiffness.

Authors - Yueting Peng
  • KD Validated
humanWB

Cancer Med

Effects of RhoC downregulation on the angiogenesis characteristics of myeloma vascular endothelial cells.

Authors - Zhihua Zhao
mouseWB

Mol Hum Reprod

RAB35 depletion affects spindle formation and actin-based spindle migration in mouse oocyte meiosis.

Authors - Yu Zhang
ratWB

Oxid Med Cell Longev

Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates.

Authors - Mengyang Zhang
ratWB,IHC

Andrologia

Androgen deficiency impairs erectile function in rats through promotion of corporal fibrosis.

Authors - K Cui