|Positive WB detected in||HeLa cells, Jurkat cells|
|Positive IHC detected in||human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21850-1-AP is ROCK1 Fusion Protein expressed in E. coli.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ROCK1 fusion protein Ag16317|
|Full Name||Rho-associated, coiled-coil containing protein kinase 1|
|Calculated molecular weight||1354 aa, 158 kDa|
|Observed molecular weight||150-160 kDa|
|GenBank accession number||BC113114|
|Gene ID (NCBI)||6093|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.
J Cell Biochem
Chondroitin sulfate proteoglycan represses neural stem/progenitor cells migration via PTPσ/α-actinin4 signaling pathway.
ROCK isoforms differentially modulate cancer cell motility by mechanosensing the substrate stiffness.
Effects of RhoC downregulation on the angiogenesis characteristics of myeloma vascular endothelial cells.
Mol Hum Reprod
RAB35 depletion affects spindle formation and actin-based spindle migration in mouse oocyte meiosis.
Androgen deficiency impairs erectile function in rats through promotion of corporal fibrosis.
miR-150 is downregulated in osteosarcoma and suppresses cell proliferation, migration and invasion by targeting ROCK1.