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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated ROCK1 Polyclonal antibody

ROCK1 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-21850

Synonyms

p160 ROCK 1, P160ROCK, PRO0435, ROCK1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated ROCK1 Polyclonal antibody (CL594-21850)

    IF Staining of HeLa using CL594-21850

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 ROCK1 antibody (CL594-21850) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated ROCK1 Polyclonal antibody (CL594-21850)

    FC experiment of HeLa using CL594-21850

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ROCK1 (CL594-21850) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-21850 is ROCK1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ROCK1 fusion protein Ag16317
Full Name Rho-associated, coiled-coil containing protein kinase 1
Calculated Molecular Weight 1354 aa, 158 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC113114
Gene Symbol ROCK1
Gene ID (NCBI) 6093
RRIDAB_2919864
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.

Protocols

Product Specific Protocols
IF protocol for CL594 ROCK1 antibody CL594-21850Download protocol
FC protocol for CL594 ROCK1 antibody CL594-21850Download protocol
Standard Protocols
Click here to view our Standard Protocols