• Featured Product
  • KD/KO Validated

ROCK1-Specific Polyclonal antibody

ROCK1-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 20247-1-AP


p160 ROCK 1, P160ROCK, PRO0435, ROCK1, ROCK1-Specific

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20247-1-AP targets ROCK1-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Rho-associated, coiled-coil containing protein kinase 1
Calculated Molecular Weight 158 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberNM_005406
Gene Symbol ROCK1
Gene ID (NCBI) 6093
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to ROCK1.


Product Specific Protocols
WB protocol for ROCK1-Specific antibody 20247-1-APDownload protocol
IHC protocol for ROCK1-Specific antibody 20247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



ASN Neuro

Induction of oligodendrocyte differentiation and in vitro myelination by inhibition of rho-associated kinase.

Authors - Carlos E Pedraza
  • KD Validated