ROCK1-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

ROCK1-Specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20247-1-AP

Synonyms

p160 ROCK 1, P160ROCK, PRO0435, ROCK1, ROCK1-Specific

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Validation Data Gallery

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  WB analysis of HeLa using 20247-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20247-1-AP (ROCK1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 20247-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20247-1-AP (ROCK1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

20247-1-AP targets ROCK1-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Rho-associated, coiled-coil containing protein kinase 1
• Calculated Molecular Weight: 158 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: NM_005406
• Gene Symbol: ROCK1
• Gene ID (NCBI): 6093
• RRID: AB_10694275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to ROCK1.

Publications

Species	Application	Title
mouse	WB	ASN Neuro; Induction of oligodendrocyte differentiation and in vitro myelination by inhibition of rho-associated kinase.; Authors - Carlos E Pedraza ; KD Validated