Tested Applications
| Positive WB detected in | HeLa cells, PC-3 cells, Y79 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83861-1-RR targets RPE65 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag12356 Product name: Recombinant human RPE65 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 204-533 aa of BC075036 Sequence: YNIVKIPPLQADKEDPISKSEIVVQFPCSDRFKPSYVHSFGLTPNYIVFVETPVKINLFKFLSSWSLWGANYMDCFESNETMGVWLHIADKKRKKYLNNKYRTSPFNLFHHINTYEDNGFLIVDLCCWKGFEFVYNYLYLANLRENWEEVKKNARKAPQPEVRRYVLPLNIDKADTGKNLVTLPNTTATAILCSDETIWLEPEVLFSGPRQAFEFPQINYQKYCGKPYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPAYLLILNAKDLSEVARAEVEINIPVTFHGLFKKS Predict reactive species |
| Full Name | retinal pigment epithelium-specific protein 65kDa |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 65 kDa, 61 kDa |
| GenBank Accession Number | BC075036 |
| Gene Symbol | RPE65 |
| Gene ID (NCBI) | 6121 |
| RRID | AB_3671445 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16518 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RPE65(Retinal pigment epithelium-specific 65 kDa protein) is also named as retinoid isomerohydrolase with the calculated molecular weight og 61 kDa and belongs to the carotenoid oxygenase family. It is a microsomal protein with isomerase activity in the retinoid visual cycle that playing a role in the isomerisation of all-trans (vitamin A) to 11-cis retinal, the chromophore of the visual pigments. It also might be involved in retinoid uptake of keratinocytes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPE65 antibody 83861-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPE65 recombinant monoclonal antibody (catalog number 83861-1-RR) has 1 total citation. This citation appears in the journal Neuropharmacology, published in 2026. The associated research field is neuropharmacology, specifically investigating the adenosine A2A receptor antagonist KW6002 and its role in mitigating aldosterone-induced central serous chorioretinopathy in mice. This study connects RPE65 function to retinal pigment epithelium biology and retinoid visual cycle pathways within a neuropharmacological disease model.
Word count: 85.
| Species | Application | Title |
|---|---|---|
Neuropharmacology The adenosine A(2A) receptor antagonist KW6002 mitigates aldosterone-induced central serous chorioretinopathy in mice. |
Reviews
What customers say
The single review reports using this RPE65 recombinant antibody for Western blotting at a 1:5000 dilution on ARPE-19 cells. The user observed clear bands with minimal non-specific signal at higher molecular weights, but noted the detected band appeared at approximately 95 kDa rather than the expected ~65 kDa. Overall, the reviewer rated the product 2 out of 5, reflecting concern about the unexpected band size despite acceptable specificity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Alex (Verified Customer) (12-16-2024) | I have tested this RPE65 primary recombinant antibody used in western blotting, dilution factor 1 in 5000 as recommended in the website, used on ARPE-19 cells. I have clear bands with minimum non-specific bands at higher molecular weight, however, the clear bands correspond to ~95 kDa and not ~65 kDa.
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