RPL7A Recombinant monoclonal antibody

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  WB analysis using 86270-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86270-4-RR (RPL7A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 86270-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL7A antibody (86270-4-RR, Clone: 250886G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RPL7A Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86270-4-RR

Clone No.250886G9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

60S ribosomal protein L7a, Large ribosomal subunit protein eL8, PLA X polypeptide, PLA-X polypeptide, ribosomal protein L7a

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, Jurkat cells, K-562 cells, RAW 264.7 cells, mouse kidney tissue, rat kidney tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86270-4-RR targets RPL7A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7543

  Product name: Recombinant human RPL7A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-266 aa of BC005128

  Sequence: MPKGKKAKGKKVAPAPAVVKKQEAKKVVNPLFEKRPKNFGIGQDIQPKRDLTRFVKWPRYIRLQRQRAILYKRLKVPPAINQFTQALDRQTATQLLKLAHKYRPETKQEKKQRLLARAEKKAAGKGDVPTKRPPVLRAGVNTVTTLVENKKAQLVVIAHDVDPIELVVFLPALCRKMGVPYCIIKGKARLGRLVHRKTCTTVAFTQVNSEDKGALAKLVEAIRTNYNDRYDEIRRHWGGNVLGPKSVARIAKLEKAKAKELATKLG

• Full Name: ribosomal protein L7a
• Calculated Molecular Weight: 266 aa, 30 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC005128
• Gene Symbol: RPL7A
• Gene ID (NCBI): 6130
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62424
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal proteins are a major component of ribosomes, which catalyze protein synthesis. RPL7a, which is a component of the 60S large ribosomal subunit, has additional functions involved in cell growth and differentiation that occur via interaction with human thyroid hormone receptor (THR) and retinoic acid receptor (RAR) and in turn inhibit the activities of the two nuclear hormone receptors [PMID:21505254].