CoraLite® Plus 488-conjugated RPL7A Recombinant monoclonal antibody

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  IF Staining of HeLa using CL488-86270-4

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 RPL7A antibody (CL488-86270-4, Clone: 250886G9 ) at dilution of 1:800, CL594-Phalloidin (red).

RPL7A Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-86270-4

Clone No.250886G9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

60S ribosomal protein L7a, Large ribosomal subunit protein eL8, PLA X polypeptide, PLA-X polypeptide, ribosomal protein L7a

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-86270-4 targets RPL7A in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7543

  Product name: Recombinant human RPL7A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-266 aa of BC005128

  Sequence: MPKGKKAKGKKVAPAPAVVKKQEAKKVVNPLFEKRPKNFGIGQDIQPKRDLTRFVKWPRYIRLQRQRAILYKRLKVPPAINQFTQALDRQTATQLLKLAHKYRPETKQEKKQRLLARAEKKAAGKGDVPTKRPPVLRAGVNTVTTLVENKKAQLVVIAHDVDPIELVVFLPALCRKMGVPYCIIKGKARLGRLVHRKTCTTVAFTQVNSEDKGALAKLVEAIRTNYNDRYDEIRRHWGGNVLGPKSVARIAKLEKAKAKELATKLG

• Full Name: ribosomal protein L7a
• Calculated Molecular Weight: 266 aa, 30 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC005128
• Gene Symbol: RPL7A
• Gene ID (NCBI): 6130
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62424
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ribosomal proteins are a major component of ribosomes, which catalyze protein synthesis. RPL7a, which is a component of the 60S large ribosomal subunit, has additional functions involved in cell growth and differentiation that occur via interaction with human thyroid hormone receptor (THR) and retinoic acid receptor (RAR) and in turn inhibit the activities of the two nuclear hormone receptors [PMID:21505254].