RPRD1B Polyclonal antibody, PBS Only

Name: RPRD1B Polyclonal antibody, PBS Only,
Brand: Proteintech

RPRD1B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 24542-1-PBS

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C20orf77, Cell cycle-related and expression-elevated protein in tumor, CREPT, Regulation of nuclear pre-mRNA domain-containing protein 1B

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis using 24542-1-AP (same clone as 24542-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 24542-1-AP (RPRD1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24542-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 24542-1-AP (same clone as 24542-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 24542-1-AP (RPRD1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24542-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 24542-1-AP (same clone as 24542-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24542-1-AP (RPRD1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24542-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human cervical cancer using 24542-1-AP (same clone as 24542-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24542-1-AP (RPRD1B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24542-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 24542-1-AP (same clone as 24542-1-PBS)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 24542-1-AP (RPRD1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24542-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications
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Product Information

24542-1-PBS targets RPRD1B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag21578 Product name: Recombinant human RPRD1B protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 79-181 aa of BC033629 Sequence: REFESVLVDAFSHVAREADEGCKKPLERLLNIWQERSVYGGEFIQQLKLSMEDSKSPPPKATEEKKSLKRTFQQIQEEEDDDYPGSYSPQDPSAGPLLTEELI Predict reactive species
Full Name	regulation of nuclear pre-mRNA domain containing 1B
Calculated Molecular Weight	326 aa, 37 kDa
Observed Molecular Weight	37 kDa
GenBank Accession Number	BC033629
Gene Symbol	RPRD1B
Gene ID (NCBI)	58490
RRID	AB_2879597
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NQG5
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

RPRD1B, also known as CREPT, is a protein-coding gene located on chromosome 20q11.23 that encodes a 326-amino-acid nuclear protein. RPRD1B functions primarily as a scaffold for the C-terminal domain (CTD) of RNA polymerase II (RNAPII), interacting with phosphorylated CTD repeats via its CTD-interaction domain (CID) and coordinating the dephosphorylation of phospho-Ser5 by the RPAP2 phosphatase. It forms homodimers and heterodimers with its paralog RPRD1A through coiled-coil domains and participates in transcription-coupled RNA processing and RNAPII promoter clearance. Functionally, RPRD1B enhances the expression of cell cycle-related genes, notably cyclin D1 (CCND1), by promoting RNAPII binding to the CCND1 promoter and facilitating loop formation that may enable RNAPII recycling during transcription. Beyond its physiological roles in transcription regulation and cell proliferation, RPRD1B is frequently overexpressed across multiple cancer types and has been shown to promote tumorigenesis by accelerating cell cycle progression, enhancing proliferative signaling, and supporting cancer cell migration and invasion, positioning it as a promising diagnostic and prognostic biomarker.