RYR1 Monoclonal antibody, PBS Only

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  IHC staining of mouse skeletal muscle using 66539-1-Ig (same clone as 66539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66539-1-Ig (RYR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66539-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 66539-1-Ig (same clone as 66539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66539-1-Ig (RYR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66539-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66539-1-Ig (same clone as 66539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66539-1-Ig (RYR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66539-1-PBS in a different storage buffer formulation.

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  IF Staining of A-673 using 66539-1-Ig (same clone as 66539-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A-673 cells using RYR1 antibody (66539-1-Ig, Clone: 1B4E12 ) at dilution of 1:300 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66539-1-PBS in a different storage buffer formulation.

RYR1 Monoclonal Antibody for IHC, IF/ICC, Indirect ELISA

Cat No. 66539-1-PBS

Clone No.1B4E12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

1B4E12, CCO, Ryanodine receptor 1, RYDR, RYR1/2/3

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Product Information

66539-1-PBS targets RYR1 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25120

  Product name: Recombinant human RYR1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 4942-5038 aa of NM_000540

  Sequence: GELRDQQEQVKEDMETKCFICGIGSDYFDTTPHGFETHTLEEHNLANYMFFLMYLINKDETEHTGQESYVWKMYQERCWDFFPAGDCFRKQYEDQLS

• Full Name: ryanodine receptor 1 (skeletal)
• Calculated Molecular Weight: 565 kDa
• GenBank Accession Number: NM_000540
• Gene Symbol: RYR1
• Gene ID (NCBI): 6261
• RRID: AB_2881901
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21817
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Ryanodine receptor isoform-1 (RyR1) is a major calcium channel in skeletal muscle important for excitation-contraction coupling. The transmembrane region of RyR1 is comprised of two domains including the pseudo voltage sensor domain and the pore-forming domain (PMID: 27855725). Dominant RyR1 mutations are the leading cause of MH. Studies on the Ca2+ conducting properties of RyR1 channels containing Malignant hyperthermia mutations expressed in myotubes.