Raptor Polyclonal antibody, PBS Only

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• Raptor Polyclonal antibody, PBS Only (20984-1-PBS)

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  WB analysis using 20984-1-AP (same clone as 20984-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20984-1-AP (Raptor antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 cells using 20984-1-AP (same clone as 20984-1-PBS)

  WB result of Raptor antibody (20984-1-AP, 1:500) with si-control and si-Raptor transfected HepG2 cells. This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 20984-1-AP (same clone as 20984-1-PBS)

  IP result of anti-Raptor (IP:20984-1-AP, 4ug; Detection:20984-1-AP 1:4000) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocellular carcinoma using 20984-1-AP (same clone as 20984-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma slide using 20984-1-AP (Raptor antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 20984-1-AP (same clone as 20984-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Raptor antibody (20984-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Raptor Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 20984-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

KIAA1303, p150 target of rapamycin, Regulatory-associated protein of mTOR, RPTOR, TOR

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Product Information

20984-1-PBS targets Raptor in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: raptor
• Calculated Molecular Weight: 149 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: NM_020761
• Gene Symbol: Raptor
• Gene ID (NCBI): 57521
• RRID: AB_11182390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N122
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RPTOR, also named as KIAA1303 and RAPTOR Belongs to the WD repeat RAPTOR family. It is involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. The antibody is specific to RPTOR.