S100A10 Monoclonal antibody

S100A10 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1B3F9

Cat no : 66227-1-Ig

Synonyms

42C, ANX2L, ANX2LG, Ca[1], CAL1L, Calpactin 1 light chain, Calpactin I light chain, Cellular ligand of annexin II, CLP11, GP11, p10, p10 protein, P11, Protein S100 A10, S100A10



Tested Applications

Positive WB detected inhuman lung tissue
Positive IHC detected inhuman colon cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66227-1-Ig targets S100A10 in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen S100A10 fusion protein Ag23596
Full Name S100 calcium binding protein A10
Calculated molecular weight 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC015973
Gene symbol S100A10
Gene ID (NCBI) 6281
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.

Protocols

Product Specific Protocols
WB protocol for S100A10 antibody 66227-1-IgDownload protocol
IHC protocol for S100A10 antibody 66227-1-IgDownload protocol
IF protocol for S100A10 antibody 66227-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols