CoraLite® Plus 488-conjugated S100A10 Monoclonal antibody

S100A10 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B3F9

Cat no : CL488-66227

Synonyms

42C, ANX2L, ANX2LG, Ca[1], CAL1L, Calpactin 1 light chain, Calpactin I light chain, Cellular ligand of annexin II, CLP11, GP11, p10, p10 protein, P11, Protein S100 A10, S100A10

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Validation Data Gallery

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  IF Staining of A431 using CL488-66227

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®®488 S100A10 antibody (CL488-66227, Clone: 1B3F9 ) at dilution of 1:200, CL594-Phalloidin (red).

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  IF Staining of Saos-2 using CL488-66227

  Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using CoraLite® Plus 488 S100A10 antibody (CL488-66227, Clone: 1B3F9 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: A431 cells, Saos-2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66227 targets S100A10 in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: S100A10 fusion protein Ag23596
• Full Name: S100 calcium binding protein A10
• Calculated Molecular Weight: 11 kDa
• GenBank Accession Number: BC015973
• Gene Symbol: S100A10
• Gene ID (NCBI): 6281
• RRID: AB_2919303
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.