S100A13 Polyclonal antibody

• Featured Product
• KD/KO Validated

S100A13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 14987-1-AP

Synonyms

S100 calcium-binding protein A13, Protein S100-A13, Protein S100 A13

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Validation Data Gallery

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  WB analysis of MCF-7 using 14987-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14987-1-AP (S100A13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14987-1-AP (S100A13 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human thyroid cancer using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14987-1-AP (S100A13 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14987-1-AP (S100A13 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14987-1-AP (S100A13 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14987-1-AP (S100A13 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14987-1-AP (S100A13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14987-1-AP (S100A13 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14987-1-AP (S100A13 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14987-1-AP (S100A13 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14987-1-AP (S100A13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14987-1-AP (S100A13 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14987-1-AP (S100A13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14987-1-AP (S100A13 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14987-1-AP (S100A13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14987-1-AP (S100A13 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14987-1-AP (S100A13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14987-1-AP (S100A13 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14987-1-AP (S100A13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14987-1-AP (S100A13 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14987-1-AP (S100A13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human thyroid using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 14987-1-AP (S100A13 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human thyroid using 14987-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 14987-1-AP (S100A13 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 14987-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 14987-1-AP (S100A13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human thyroid cancer tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue, human thyroid tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Product Information

14987-1-AP targets S100A13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: S100A13 fusion protein Ag6971
• Full Name: S100 calcium binding protein A13
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC000632
• Gene Symbol: S100A13
• Gene ID (NCBI): 6284
• RRID: AB_2878097
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Gastroenterology; Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors; Authors - Mingjun Sun
human	IHC,WB	J Oral Pathol Med; Systematic screening identifies a TEAD4-S100A13 axis modulating cisplatin sensitivity of oral squamous cell carcinoma cells.; Authors - Ruicen Li ; KD Validated
human	WB,IHC	Front Immunol; Identification of a novel immune-related gene signature for prognosis and the tumor microenvironment in patients with uveal melanoma combining single-cell and bulk sequencing data; Authors - Wanpeng Wang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guorong (Verified Customer) (11-22-2022)

No bands were observed. Does not represent the performance in other kind of samples

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ARPE-19