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S100A14 Polyclonal antibody

S100A14 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10489-1-AP

Synonyms

Protein S100 A14, S100A14, S100A15, S114



Tested Applications

Positive WB detected inMCF-7 cells, rat stomach tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human gliomas tissue, human ovary tumor tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10489-1-AP targets S100A14 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A14 fusion protein Ag0757
Full Name S100 calcium binding protein A14
Calculated molecular weight 12 kDa
Observed molecular weight 10-12 kDa
GenBank accession numberBC005019
Gene symbol S100A14
Gene ID (NCBI) 57402
RRIDAB_2183628
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).

Protocols

Product Specific Protocols
WB protocol for S100A14 antibody 10489-1-APDownload protocol
IHC protocol for S100A14 antibody 10489-1-APDownload protocol
IF protocol for S100A14 antibody 10489-1-APDownload protocol
IP protocol for S100A14 antibody 10489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Oncotarget

The role of S100A14 in epithelial ovarian tumors.

Authors - Hanbyoul Cho
humanWB, IHC

Oncogene

S100A14 suppresses metastasis of nasopharyngeal carcinoma by inhibition of NF-kB signaling through degradation of IRAK1.

Authors - Dong-Fang Meng
humanWB, IHC

Int J Cancer

S100A14 is a novel independent prognostic biomarker in the triple-negative breast cancer subtype.

Authors - Sidse Ehmsen
humanWB,IHC,IF

Cell Death Dis

Calcium-binding protein S100A14 induces differentiation and suppresses metastasis in gastric cancer.

Authors - Min Zhu
humanIHC,WB

Am J Cancer Res

A new 7-gene survival score assay for pancreatic cancer patient prognosis prediction.

Authors - Lisi Luo
humanWB,IHC

Eur J Cancer

S100A14 regulates the invasive potential of oral squamous cell carcinoma derived cell-lines in vitro by modulating expression of matrix metalloproteinases, MMP1 and MMP9.

Authors - Sapkota Dipak D
  • KD Validated