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  • KD/KO Validated

S100A14 Polyclonal antibody

S100A14 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10489-1-AP

Synonyms

Protein S100 A14, S100A14, S100A15, S114



Tested Applications

Positive WB detected inMCF-7 cells, rat stomach tissue
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human gliomas tissue, human ovary tumor tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10489-1-AP targets S100A14 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A14 fusion protein Ag0757
Full Name S100 calcium binding protein A14
Calculated molecular weight 12 kDa
Observed molecular weight 10-12 kDa
GenBank accession numberBC005019
Gene symbol S100A14
Gene ID (NCBI) 57402
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).

Protocols

Product Specific Protocols
WB protocol for S100A14 antibody 10489-1-APDownload protocol
IHC protocol for S100A14 antibody 10489-1-APDownload protocol
IF protocol for S100A14 antibody 10489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Genomics Proteomics

Profiling of Chromosomal Changes in Potentially Malignant and Malignant Oral Mucosal Lesions from South and South-East Asia Using Array-comparative Genomic Hybridization.

Authors - Mai Lill Suhr Lunde
humanWB,IHC

Thorac Cancer

Overexpression of S100A14 contributes to malignant progression and predicts poor prognosis of lung adenocarcinoma.

Authors - Fang Ding
  • KD Validated
humanWB

Biochem Biophys Rep

SOX2 suppresses the mobility of urothelial carcinoma by promoting the expression of S100A14.

Authors - Moon-Sing Lee
humanIF

J Assist Reprod Genet

Differentially expressed genes in preimplantation human embryos: potential candidate genes for blastocyst formation and implantation.

Authors - Erika M Munch
humanWB

Mol Med Rep

S100A16 suppresses the proliferation, migration and invasion of colorectal cancer cells in part via the JNK/p38 MAPK pathway.

Authors - Shiyu Ou
humanWB,IHC

Head Neck

Loss of S100A14 expression at the tumor-invading front correlates with poor differentiation and worse prognosis in oral squamous cell carcinoma.

Authors - Sushma Pandey
  • KD Validated