|Positive WB detected in||MCF-7 cells, rat stomach tissue|
|Positive IHC detected in||human breast cancer tissue, human colon cancer tissue, human gliomas tissue, human ovary tumor tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10489-1-AP targets S100A14 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||S100A14 fusion protein Ag0757|
|Full Name||S100 calcium binding protein A14|
|Calculated molecular weight||12 kDa|
|Observed molecular weight||10-12 kDa|
|GenBank accession number||BC005019|
|Gene ID (NCBI)||57402|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).
Cancer Genomics Proteomics
Profiling of Chromosomal Changes in Potentially Malignant and Malignant Oral Mucosal Lesions from South and South-East Asia Using Array-comparative Genomic Hybridization.
Overexpression of S100A14 contributes to malignant progression and predicts poor prognosis of lung adenocarcinoma.
Biochem Biophys Rep
SOX2 suppresses the mobility of urothelial carcinoma by promoting the expression of S100A14.
J Assist Reprod Genet
Differentially expressed genes in preimplantation human embryos: potential candidate genes for blastocyst formation and implantation.
Mol Med Rep
S100A16 suppresses the proliferation, migration and invasion of colorectal cancer cells in part via the JNK/p38 MAPK pathway.
Loss of S100A14 expression at the tumor-invading front correlates with poor differentiation and worse prognosis in oral squamous cell carcinoma.