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S100A9 Polyclonal antibody
S100A9 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 26992-1-AP
Validation Data Gallery
|Positive WB detected in||HL-60 cells, MCF-7 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue, mouse spleen tissue, human liver cancer tissue, human tonsillitis tissue, human oesophagus cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, A549 cells, HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
26992-1-AP targets S100A9 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||S100A9 fusion protein Ag25764|
|Full Name||S100 calcium binding protein A9|
|Calculated molecular weight||13 kDa|
|Observed molecular weight||14 kDa|
|GenBank accession number||BC047681|
|Gene ID (NCBI)||6280|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.
|Product Specific Protocols|
|WB protocol for S100A9 antibody 26992-1-AP||Download protocol|
|IHC protocol for S100A9 antibody 26992-1-AP||Download protocol|
|IF protocol for S100A9 antibody 26992-1-AP||Download protocol|
|IP protocol for S100A9 antibody 26992-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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