|Positive WB detected in||HL-60 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue, human liver cancer tissue, mouse spleen tissue, human tonsillitis tissue, human oesophagus cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 26992-1-AP is S100A9 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||S100A9 fusion protein Ag25764|
|Full Name||S100 calcium binding protein A9|
|Calculated molecular weight||13 kDa|
|Observed molecular weight||14 kDa|
|GenBank accession number||BC047681|
|Gene ID (NCBI)||6280|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.
|Product Specific Protocols|
|WB protocol for S100A9 antibody 26992-1-AP||Download protocol|
|IHC protocol for S100A9 antibody 26992-1-AP||Download protocol|
|IF protocol for S100A9 antibody 26992-1-AP||Download protocol|
|IP protocol for S100A9 antibody 26992-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Madridge J Diabetes
Proteomic analyses of Urine Exosomes reveal New Biomarkers of Diabetes in Pregnancy.
Acta Physiol (Oxf)
Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.
Syst Biol Reprod Med
Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts.
Arch Oral Biol
Comparative proteomic profiling of human dental pulp stem cells and periodontal ligament stem cells under in vitro osteogenic induction.
Proteomic analysis of human periodontal ligament cells under hypoxia.
Cancer Cell Int
Identification of lysine acetylome in cervical cancer by label-free quantitative proteomics.