S100A9 Polyclonal antibody

S100A9 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 26992-1-AP

Synonyms

60B8AG, CAGB, Calgranulin B, Calprotectin L1H subunit, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP 14, MRP14, NIF, P14, Protein S100 A9, S100A9



Tested Applications

Positive WB detected inHL-60 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, mouse spleen tissue, human tonsillitis tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26992-1-AP is S100A9 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A9 fusion protein Ag25764
Full Name S100 calcium binding protein A9
Calculated molecular weight 13 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC047681
Gene symbol S100A9
Gene ID (NCBI) 6280
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

Protocols

Product Specific Protocols
WB protocol for S100A9 antibody 26992-1-APDownload protocol
IHC protocol for S100A9 antibody 26992-1-APDownload protocol
IF protocol for S100A9 antibody 26992-1-APDownload protocol
IP protocol for S100A9 antibody 26992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Madridge J Diabetes

Proteomic analyses of Urine Exosomes reveal New Biomarkers of Diabetes in Pregnancy.

Authors - Satish P Ramachandrarao
ratWB

Acta Physiol (Oxf)

Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.

Authors - Li Zhang
humanIHC

Syst Biol Reprod Med

Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts.

Authors - Genhai Zhu
humanWB

Arch Oral Biol

Comparative proteomic profiling of human dental pulp stem cells and periodontal ligament stem cells under in vitro osteogenic induction.

Authors - He Wang
humanWB

Proteome Sci

Proteomic analysis of human periodontal ligament cells under hypoxia.

Authors - Qiwen Li
humanIP

Cancer Cell Int

Identification of lysine acetylome in cervical cancer by label-free quantitative proteomics.

Authors - Lu Zhang