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SAFB2 Polyclonal antibody, PBS Only (11642-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of MCF-7 using 11642-1-AP (same clone as 11642-1-PBS)
MCF-7 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.
WB analysis of rat pancreas using 11642-1-AP (same clone as 11642-1-PBS)
rat pancreas tissue were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 11642-1-AP (same clone as 11642-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 11642-1-AP (same clone as 11642-1-PBS)
MCF7 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.
WB analysis of BxPC-3 using 11642-1-AP (same clone as 11642-1-PBS)
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 11642-1-AP (same clone as 11642-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11642-1-AP (SAFB2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 11642-1-AP (same clone as 11642-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11642-1-AP (SAFB2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 11642-1-AP (same clone as 11642-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11642-1-AP (SAFB2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 11642-1-AP (same clone as 11642-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SAFB2 antibody (11642-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.

SAFB2 Polyclonal antibody, PBS Only

Name: SAFB2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 11642-1-PBS

SAFB2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 11642-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

KIAA0138, SAF B2, SAF-B2

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

11642-1-PBS targets SAFB2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2230 Product name: Recombinant human SAFB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC025279 Sequence: MAETLPGSGDSGPGTASLGPGVAETGTRRLSELRVIDLRAELKKRNLDTGGNKSVLMERLKKAVKEEGQDPDEIGIELEATSKKSAKRCVKGLKMEEEGTEDNGLEDDSRDGQEDMEASLENLQNMGMMDMSVLDETEVANSSAPDFGEDGTDGLLDSFCDSKEYVAAQLRQLPAQPPEHAVDGEGFKNTLETSSLNFKVTPDIEESLLEPENEKILDILGETCKSEPVKEESSELEQPFAQDTSSVGPDRKLAEEEDLFDSAHPEEGDLDLASESTAHAQSSKADSLLAVVKREPAEQPGDGERTDCEPVGLEPAVEQSSAASELAEASSEELAEAPTEAPSPEARDS Predict reactive species
Full Name	scaffold attachment factor B2
Calculated Molecular Weight	528aa,57 kDa; 953aa,107 kDa
Observed Molecular Weight	130 kDa
GenBank Accession Number	BC025279
Gene Symbol	SAFB2
Gene ID (NCBI)	9667
RRID	AB_2301309
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14151
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SAFB2, also named as Scaffold attachment factor B2 or KIAA0138, is a 953 amino acid protein, which contains one SAP domain and one RRM (RNA recognition motif) domain. SAFB2 expressed at high levels in the CNS and at low levels in the liver. It also is expressed in a wide number of breast cancer cell lines. SAFB2 binds to scaffold/matrix attachment region (S/MAR) DNA and can function as an estrogen receptor corepressor and can also inhibit cell proliferation.