SAGE1 Polyclonal antibody, PBS Only

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• SAGE1 Polyclonal antibody, PBS Only (30914-1-PBS)

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  IHC staining of human skin cancer using 30914-1-AP (same clone as 30914-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 30914-1-AP (SAGE1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30914-1-PBS in a different storage buffer formulation.

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  IF Staining of A375 using 30914-1-AP (same clone as 30914-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using SAGE1 antibody (30914-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30914-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 30914-1-AP (same clone as 30914-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SAGE1 antibody (30914-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30914-1-PBS in a different storage buffer formulation.

SAGE1 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Cat No. 30914-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

Cancer/testis antigen 14, CT14, SAGE, sarcoma antigen 1

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Product Information

30914-1-PBS targets SAGE1 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34216

  Product name: Recombinant human SAGE1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 247-387 aa of NM_018666.2

  Sequence: ATITYNVPEEKMEKGQPQPDNILSTASTGLINVAGAGTPAISTNGLYSTVPHNVCEEKMENDQPQPNNVLSTVQPVIIYLTATGIPGMNTRDQYATITHNVCEERVVNNQPLPSNALSTVLPGLAYLATADMPAMSTRDQH

• Full Name: sarcoma antigen 1
• Calculated Molecular Weight: 99kd
• GenBank Accession Number: NM_018666.2
• Gene Symbol: SAGE1
• Gene ID (NCBI): 55511
• RRID: AB_3669783
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9NXZ1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596). It is primarily found in primates and is generally absent in lower mammals like rodents