1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Sage1 Recombinant Antibody (CL488-82876, Clone:230094C11)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Sage1 Recombinant Antibody (CL488-82876, Clone:230094C11)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage Conditions
Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Background Information
Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596).
Protocols
Product Specific Protocols
FC protocol for CL Plus 488 SAGE1 antibody CL488-82876
1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Sage1 Recombinant Antibody (CL488-82876, Clone:230094C11)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
SAGE1 Recombinant antibody
Catalog Number
CL488-82876
Citations
-
Dilutions
FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Applications
FC (Intra)
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
