CoraLite® Plus 488-conjugated SAGE1 Recombinant monoclonal antibody

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  FC experiment of U2OS using CL488-82876

  1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Sage1 Recombinant Antibody (CL488-82876, Clone:230094C11)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

SAGE1 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL488-82876

Clone No.230094C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

sarcoma antigen 1, SAGE, CT14, Cancer/testis antigen 14, 230094C11

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Tested Applications

• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82876 targets SAGE1 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag34216

  Product name: Recombinant human SAGE1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 247-387 aa of NM_018666.2

  Sequence: ATITYNVPEEKMEKGQPQPDNILSTASTGLINVAGAGTPAISTNGLYSTVPHNVCEEKMENDQPQPNNVLSTVQPVIIYLTATGIPGMNTRDQYATITHNVCEERVVNNQPLPSNALSTVLPGLAYLATADMPAMSTRDQH

• Full Name: sarcoma antigen 1
• Calculated Molecular Weight: 99kd
• GenBank Accession Number: NM_018666.2
• Gene Symbol: SAGE1
• Gene ID (NCBI): 55511
• RRID: AB_3673092
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NXZ1
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596).