SAMD14 Polyclonal antibody

SAMD14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 22009-1-AP

Synonyms

Sterile alpha motif domain-containing protein 14, SAM domain-containing protein 14

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Validation Data Gallery

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  WB analysis using 22009-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22009-1-AP (SAMD14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 22009-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22009-1-AP (SAMD14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 22009-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22009-1-AP (SAMD14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 22009-1-AP

  Immunofluorescent analysis of HepG2 cells using 22009-1-AP (SAMD14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 22009-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug SAMD14 Polyclonal antibody (22009-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HEK-293 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22009-1-AP targets SAMD14 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAMD14 fusion protein Ag15653
• Full Name: sterile alpha motif domain containing 14
• Calculated Molecular Weight: 445 aa, 48 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC141822
• Gene Symbol: SAMD14
• Gene ID (NCBI): 201191
• RRID: AB_2878969
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.