SCP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SCP2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 23006-1-AP

Synonyms

SCP2/SCPx, NSL-TP, Non-specific lipid-transfer protein, EC:2.3.1.176, EC:2.3.1.16

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Validation Data Gallery

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  WB analysis using 23006-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23006-1-AP (SCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23006-1-AP

  WB result of SCP2 antibody (23006-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCP2 transfected HEK-293 cells.

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  WB analysis of K-562 using 23006-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23006-1-AP (SCP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 23006-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23006-1-AP (SCP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 23006-1-AP

  IP result of anti-SCP2 (IP:23006-1-AP, 4ug; Detection:23006-1-AP 1:500) with HepG2 cells lysate 3600ug.

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  IF Staining of HepG2 using 23006-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 23006-1-AP (SCP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 23006-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SCP2 (23006-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HEK-293 cells, K-562 cells, NIH/3T3 cells, HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

23006-1-AP targets SCP2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCP2 fusion protein Ag19215
• Full Name: sterol carrier protein 2
• Calculated Molecular Weight: 547 aa, 59 kDa
• Observed Molecular Weight: 13-15 kDa
• GenBank Accession Number: BC005911
• Gene Symbol: SCP2
• Gene ID (NCBI): 6342
• RRID: AB_2879197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCP2 (Sterol carrier protein-2), also called nonspecific lipid-transfer protein, is thought to play a major role in intracellular lipid transport and metabolism. Mutations of SCP2 have been associated with diseases involving abnormalities in lipid trafficking, such as Zellweger syndrome. The alternative splicing generates 58 kDa SCPx and 13-15 kDa SCP2 proteins, both of which contain a C-terninal SCP2 domain. In addition to the SCP2 domain, SCPx also contains an amino-terminal thiolase domain. The proteolytic cleavage of SCPx occurred when it was imported into peroxisomes, giving rise to a 44-46 kDa thiolase and a 13-15 kDa SCP2. 23006-1-AP antibody recognizes the 13-15 kDa SCP2 protein.

Publications

Species	Application	Title
rat	WB	Phytomedicine; Podophyllotoxin via SIRT1/PPAR /NF-κB axis induced cardiac injury in rats based on the toxicological evidence chain (TEC) concept; Authors - Tao Jiang
mouse	WB	Commun Biol; Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism; Authors - Michael Landowski
human	WB	iScience; A PEROXO-Tag Enables Rapid Isolation of Peroxisomes from Human Cells.; Authors - G Jordan Ray
mouse	WB	J Biol Chem; Sterol carrier protein-2 deficiency attenuates diet-induced dyslipidemia and atherosclerosis in mice.; Authors - Honglaing He ; KO Validated
mouse	IF	PLoS One; Sterol Carrier Protein-2, a Nonspecific Lipid-Transfer Protein, in Intracellular Cholesterol Trafficking in Testicular Leydig Cells.; Authors - Nancy C Li
human	WB	J Biochem; Human SCP4 is a chromatin-associated CTD phosphatase and exhibits the dynamic translocation during erythroid differentiation.; Authors - Shotaro Wani