SDCCAG8 Polyclonal antibody, PBS Only

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• SDCCAG8 Polyclonal antibody, PBS Only (13471-1-PBS)

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  IHC staining of mouse brain using 13471-1-AP (same clone as 13471-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13471-1-AP (SDCCAG8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13471-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13471-1-AP (same clone as 13471-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13471-1-AP (SDCCAG8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13471-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13471-1-AP (same clone as 13471-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SDCCAG8 antibody (13471-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13471-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SDCCAG8 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Cat No. 13471-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine, zebrafish

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

NPHP10, HSPC085, hCCCAP, CCCAP, BBS16

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Product Information

13471-1-PBS targets SDCCAG8 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, canine, zebrafish samples.

• Tested Reactivity: human, mouse, rat, canine, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4264

  Product name: Recombinant human SDCCAG8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-360 aa of BC032454

  Sequence: MAKSPENSTLEEILGQYQRSLREHASRSIHQLTCALKEGDVTIGEDAPNLSFSTSVGNEDARTAWPELQQSHAVNQLKDLLRQQADKESEVSPSRRRKMSPLRSLEHEETNMPTMHDLVHTINDQSQYIHHLEAEVKFCKEELSGMKNKIQVVVLENEGLQQQLKSQRQEETLREQTLLDASGNMHNSWITTGEDSGVGETSKRPFSHDNADFGKAASAGEQLELEKLKLTYEEKCEIEESQLKFLRNDLAEYQRTCEDLKEQLKHKEFLLAANTCNRVGGLCLKCAQHEAVLSQTHTNVHMQTIERLVKERDDLMSALVSVRSSLADTQQREASAYEQVKQVLQISEEANFEKTKHPSQ

• Full Name: serologically defined colon cancer antigen 8
• Calculated Molecular Weight: 713 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC032454
• Gene Symbol: SDCCAG8
• Gene ID (NCBI): 10806
• RRID: AB_2183417
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86SQ7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SDCCAG8, also named as CCCAP and NY-CO-8, plays a role in the establishment of cell polarity and epithelial lumen formation. It may play a role in ciliogenesis. Loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders. SDCCAG8 is localized at both centrioles and interacts directly with OFD1 (oral-facial-digital syndrome 1), which is associated with NPHP-RC. SDCCAG8 has 4 isoforms with MW 83,78,73 and 41 kDa (refer to UniProt). Catalog#13471-1-AP can recognize all the four isoforms.