SEC22B Recombinant antibody, PBS Only

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  WB analysis using 85037-5-RR (same clone as 85037-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85037-5-RR (SEC22B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85037-5-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 85037-5-RR (same clone as 85037-5-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SEC22B Recombinant antibody (85037-5-RR, Clone:242659B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85037-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85037-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85037-5-RR against Human SEC22B were performed. The affinity constant is 2.07 nM.

SEC22B Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 85037-5-PBS

Clone No.242659B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

242659B3, ER-Golgi SNARE of 24 kDa, ERS24, ERS-24, SEC22 vesicle-trafficking protein homolog B

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Product Information

85037-5-PBS targets SEC22B in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

• Tested Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SEC22B fusion protein Ag12981
• Full Name: SEC22 vesicle trafficking protein homolog B (S. cerevisiae)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC001364
• Gene Symbol: SEC22B
• Gene ID (NCBI): 9554
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75396
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) proteins are critical components of the membrane fusion machinery (PMID: 20163564). SEC22B is a SNARE involved in endoplasmic reticulum (ER)/Golgi membrane trafficking. SEC22B localizes to the ER-Golgi intermediate compartment (ERGIC) and has been identified as a regulator of antigen crosspresentation (PMID: 22153078).